부산대학교 도서관

Arbues, R.; Flores, Q.C.; Fernandez, J.A.; Yanez, E.: Caracterizacion del perfil citologico de la mucosa vaginal durante el ciclo estral en ovejas Santa Ines. Rev. Vet. 33: 2, 202-207, 2022. El objetivo de trabajo fue caracterizar el ciclo del epitelio vaginal de ovejas de raza Santa Ines, mediante estudio citologico. Se tomaron muestras de la mucosa vaginal a 10 borregas sexualmente maduras, desde el primer dia de celo y por 18 dias. Las muestras se colorearon con Papanicolaou y se observaron en microscopio optico. Se reconocieron celulas parabasales, intermedias profundas, intermedias superficiales y superficiales. Las celulas parabasales presentaron forma redondeada, citoplasma escaso y nucleo grande, fueron escasas. Las intermedias profundas de bordes redondeados, citoplasma mas abundante y nucleo menor. Se observaron en mayor cantidad que las parabasales. Las intermedias superficiales fueron las mas abundantes en todo el estudio y las de mayor diametro. Presentaron forma poligonal y nucleo mas pequeno. La identification de dos tipos de celula intermedia, coincide con trabajos en perras, vacas y alpacas, en tanto otros trabajos describen un solo tipo. Las celulas superficiales se dividieron en dos subtipos, el A, con forma poligonal y nucleo picnotico y el B, con citoplasma mas pequeno e intensamente acidofilo y nucleo picnotico. Este ultimo no se encontro hasta el momento en otros trabajos con ovejas. Las modificaciones de los tipos celulares no reflejaron de manera precisa la fase del ciclo estral. Palabras clave: citologia, hormonas, razas tropicales. Arbues, R.; Flores, Q.C.; Fernandez, J.A.; Yanez, E.: Characterization of the citological profile of the vaginal mucous during the estral cycle in the Santa Ines sheep. Rev. Vet. 33: 2, 202-207, 2022. The objective of the work was to characterize the cycle of the vaginal epithelium of sheep of the Santa Ines breed, through a cytological study. Vaginal mucosal samples were taken from 10 sexually mature ewes, from the first day of estrus and for 18 days. The samples were stained with Papanicolaou and observed under an optical microscope. Parabasal, de epintermediate, superficial intermediate, and superficial cells were recognized. The parabasal cells presented a rounded shape, little cytoplasm, and a large nucleus, they were scarce. The deep intermediate ones with rounded edges, more abundant cytoplasm, and smaller nuclei. They were observed in greater quantity than the parabasal ones. The superficial intermediate ones were the most abundant through out the study and the one swith the largest diameter. They presented a polygonal shape and a smaller nucleus. The identification of two types of intermediate cells coincides with works on dogs, cows, and alpacas, while other works describe a single type. Superficial cells were divided into two subtypes, A, with a polygonal shape and piknotic nucleus, and B, with a smaller and intensely acidophilic cytoplasm and pyknotic nucleus. The latter has not been found so far in other works with sheep. Changes in cell types did not accurately reflect the phase of the estrous cycle. Key words: cytology, hormones, tropical race.
INTRODUCCION La citologia vaginal es un metodo de estudio simple y economico que permite la observation de varios tipos celulares de la mucosa vaginal (13). Su importancia surge por la [...]