학술논문
Integrated Morphoelectric and Transcriptomic Classification of Cortical GABAergic Cells
Document Type
Report
Author
Gouwens, Nathan W.; Sorensen, Staci A.; Baftizadeh, Fahimeh; Budzillo, Agata; Lee, Brian R.; Jarsky, Tim; Alfiler, Lauren; Baker, Katherine; Barkan, Eliza; Berry, Kyla; Bertagnolli, Darren; Bickley, Kris; Bomben, Jasmine; Braun, Thomas; Brouner, Krissy; Casper, Tamara; Crichton, Kirsten; Daigle, Tanya L.; Dalley, Rachel; de Frates, Rebecca A.; Dee, Nick; Desta, Tsega; Lee, Samuel Dingman; Dotson, Nadezhda; Egdorf, Tom; Ellingwood, Lauren; Enstrom, Rachel; Esposito, Luke; Farrell, Colin; Feng, David; Fong, Olivia; Gala, Rohan; Gamlin, Clare; Gary, Amanda; Glandon, Alexandra; Goldy, Jeff; Gorham, Melissa; Graybuck, Lucas; Gu, Hong; Hadley, Kristen; Hawrylycz, Michael J.; Henry, Alex M.; Hill, DiJon; Hupp, Madie; Kebede, Sara; Kim, Tae Kyung; Kim, Lisa; Kroll, Matthew; Lee, Changkyu; Link, Katherine E.; Mallory, Matthew; Mann, Rusty; Maxwell, Michelle; McGraw, Medea; McMillen, Delissa; Mukora, Alice; Ng, Lindsay; Ng, Lydia; Ngo, Kiet; Nicovich, Philip R.; Oldre, Aaron; Park, Daniel; Peng, Hanchuan; Penn, Osnat; Pham, Thanh; Pom, Alice; Popovic, Zoran; Potekhina, Lydia; Rajanbabu, Ramkumar; Ransford, Shea; Reid, David; Rimorin, Christine; Robertson, Miranda; Ronellenfitch, Kara; Ruiz, Augustin; Sandman, David; Smith, Kimberly; Sulc, Josef; Sunkin, Susan M.; Szafer, Aaron; Tieu, Michael; Torkelson, Amy; Trinh, Jessica; Tung, Herman; Wakeman, Wayne; Ward, Katelyn; Williams, Grace; Zhou, Zhi; Ting, Jonathan T.; Arkhipov, Anton; Sumbul, Uygar; Lein, Ed S.; Koch, Christof; Yao, Zizhen; Tasic, Bosiljka; Berg, Jim; Murphy, Gabe J.; Zeng, Hongkui
Source
Cell. Nov 12, 2020, Vol. 183 Issue 4, 935
Subject
Language
English
ISSN
0092-8674
Abstract
Keywords GABAergic interneurons; somatostatin; parvalbumin; neuronal cell type; transcriptomics; taxonomy; Patch-seq; multimodal; visual cortex Highlights * Patch-seq data obtained from >4,200 GABAergic interneurons with >500 morphologies * Comprehensive characterization of morphoelectric features of transcriptomic types * 28 interneuron met-types with congruent properties across data modalities * Different Sst met-types preferentially innervate different cortical layers Summary Neurons are frequently classified into distinct types on the basis of structural, physiological, or genetic attributes. To better constrain the definition of neuronal cell types, we characterized the transcriptomes and intrinsic physiological properties of over 4,200 mouse visual cortical GABAergic interneurons and reconstructed the local morphologies of 517 of those neurons. We find that most transcriptomic types (t-types) occupy specific laminar positions within visual cortex, and, for most types, the cells mapping to a t-type exhibit consistent electrophysiological and morphological properties. These properties display both discrete and continuous variation among t-types. Through multimodal integrated analysis, we define 28 met-types that have congruent morphological, electrophysiological, and transcriptomic properties and robust mutual predictability. We identify layer-specific axon innervation pattern as a defining feature distinguishing different met-types. These met-types represent a unified definition of cortical GABAergic interneuron types, providing a systematic framework to capture existing knowledge and bridge future analyses across different modalities. Author Affiliation: (1) Allen Institute for Brain Science, Seattle, WA 98109, USA (2) Byte Physics, Schwarzastra[sz]e 9, Berlin 12055, Germany (3) Center for Game Science, Paul G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA 98195, USA * Corresponding author Article History: Received 15 February 2020; Revised 6 July 2020; Accepted 22 September 2020 (miscellaneous) Published: November 12, 2020 (footnote)4 These authors contributed equally (footnote)5 Lead Contact Byline: Nathan W. Gouwens [nathang@alleninstitute.org] (1,4,*), Staci A. Sorensen [stacis@alleninstitute.org] (1,4,**), Fahimeh Baftizadeh (1), Agata Budzillo (1), Brian R. Lee (1), Tim Jarsky (1), Lauren Alfiler (1), Katherine Baker (1), Eliza Barkan (1), Kyla Berry (1), Darren Bertagnolli (1), Kris Bickley (1), Jasmine Bomben (1), Thomas Braun (2), Krissy Brouner (1), Tamara Casper (1), Kirsten Crichton (1), Tanya L. Daigle (1), Rachel Dalley (1), Rebecca A. de Frates (1), Nick Dee (1), Tsega Desta (1), Samuel Dingman Lee (1), Nadezhda Dotson (1), Tom Egdorf (1), Lauren Ellingwood (1), Rachel Enstrom (1), Luke Esposito (1), Colin Farrell (1), David Feng (1), Olivia Fong (1), Rohan Gala (1), Clare Gamlin (1), Amanda Gary (1), Alexandra Glandon (1), Jeff Goldy (1), Melissa Gorham (1), Lucas Graybuck (1), Hong Gu (1), Kristen Hadley (1), Michael J. Hawrylycz (1), Alex M. Henry (1), DiJon Hill (1), Madie Hupp (1), Sara Kebede (1), Tae Kyung Kim (1), Lisa Kim (1), Matthew Kroll (1), Changkyu Lee (1), Katherine E. Link (1), Matthew Mallory (1), Rusty Mann (1), Michelle Maxwell (1), Medea McGraw (1), Delissa McMillen (1), Alice Mukora (1), Lindsay Ng (1), Lydia Ng (1), Kiet Ngo (1), Philip R. Nicovich (1), Aaron Oldre (1), Daniel Park (1), Hanchuan Peng (1), Osnat Penn (1), Thanh Pham (1), Alice Pom (1), Zoran Popovic (3), Lydia Potekhina (1), Ramkumar Rajanbabu (1), Shea Ransford (1), David Reid (1), Christine Rimorin (1), Miranda Robertson (1), Kara Ronellenfitch (1), Augustin Ruiz (1), David Sandman (1), Kimberly Smith (1), Josef Sulc (1), Susan M. Sunkin (1), Aaron Szafer (1), Michael Tieu (1), Amy Torkelson (1), Jessica Trinh (1), Herman Tung (1), Wayne Wakeman (1), Katelyn Ward (1), Grace Williams (1), Zhi Zhou (1), Jonathan T. Ting (1), Anton Arkhipov (1), Uygar Sumbul (1), Ed S. Lein (1), Christof Koch (1), Zizhen Yao (1), Bosiljka Tasic (1), Jim Berg (1), Gabe J. Murphy [gabem@alleninstitute.org] (1,5,***), Hongkui Zeng (1)