학술논문
Methylation estimates the risk of precancer in HPV-infected women with discrepant results between cytology and HPV16/18 genotyping
Document Type
Report
Author
Hernández-López, Rubí; Lorincz, Attila T.; Torres-Ibarra, Leticia; Reuter, Caroline; Scibior-Bentkowska, Dorota; Warman, Rhian; Nedjai, Belinda; Mendiola-Pastrana, Indira; León-Maldonado, Leith; Rivera-Paredez, Berenice; Ramírez-Palacios, Paula; Lazcano-Ponce, Eduardo; Cuzick, Jack; Salmerón, Jorge; Lorincz, Attila; Wheeler, Cosette; Gravitt, Patti; Lazcano, Eduardo; Torres, Leticia; León, Leith; Ramírez, Paula; Rivera, Berenice; Franco, Eduardo L.; Méndez, Pablo; Hernández, Mauricio; Moscicki, Anna Barbara; Flores, Yvonne; Carmona, Enrique; Schmeler, Kathleen M.; Bishai, David; Hernández, Pilar; Hernández, Rubi; Mendiola, Indira
Source
Clinical Epigenetics. October 12, 2019, Vol. 11 Issue 1
Subject
Language
English
ISSN
1868-7075
Abstract
Author(s): Rubí Hernández-López[sup.1,2] , Attila T. Lorincz[sup.3] , Leticia Torres-Ibarra[sup.1] , Caroline Reuter[sup.3] , Dorota Scibior-Bentkowska[sup.3] , Rhian Warman[sup.3] , Belinda Nedjai[sup.3] , Indira Mendiola-Pastrana[sup.1] , Leith León-Maldonado[sup.2,4] , Berenice [...]
Background Vigilant management of women with high-risk human papillomavirus (hrHPV) is necessary in cancer screening programs. To this end, we evaluated the performance of S5 (targeting DNA methylation in HPV16, HPV18, HPV31, HPV33, and human gene EPB41L3) to predict cervical intraepithelial neoplasia grade 2 or higher (CIN2+) in a sample of hrHPV-infected women referred to colposcopy in the FRIDA Study, a large screening trial in Mexico. A nested case-control sample with women referred to colposcopy either by atypical squamous cells of undetermined significance or higher (ASCUS+) in cytology and/or positive for HPV types 16 or 18 was tested by S5. Seventy-nine cases of CIN2+ were age-matched to 237 controls without a diagnosis of CIN2+ ( Results The S5 classifier separated women with CIN2+ from Conclusions S5 testing on hrHPV-positive women significantly increased diagnostic information compared to triage by HPV16/18 plus cytology and appears to have clinical utility as an additional test to substantially lessen burdens on colposcopy. Trial registration The FRIDA Study is registered in ClinicalTrials.gov, number NCT02510027. Keywords: DNA methylation, S5 classifier, Human papillomavirus, EPB41L3, Triage, Cervical intraepithelial neoplasia, Cervical cancer
Background Vigilant management of women with high-risk human papillomavirus (hrHPV) is necessary in cancer screening programs. To this end, we evaluated the performance of S5 (targeting DNA methylation in HPV16, HPV18, HPV31, HPV33, and human gene EPB41L3) to predict cervical intraepithelial neoplasia grade 2 or higher (CIN2+) in a sample of hrHPV-infected women referred to colposcopy in the FRIDA Study, a large screening trial in Mexico. A nested case-control sample with women referred to colposcopy either by atypical squamous cells of undetermined significance or higher (ASCUS+) in cytology and/or positive for HPV types 16 or 18 was tested by S5. Seventy-nine cases of CIN2+ were age-matched to 237 controls without a diagnosis of CIN2+ ( Results The S5 classifier separated women with CIN2+ from Conclusions S5 testing on hrHPV-positive women significantly increased diagnostic information compared to triage by HPV16/18 plus cytology and appears to have clinical utility as an additional test to substantially lessen burdens on colposcopy. Trial registration The FRIDA Study is registered in ClinicalTrials.gov, number NCT02510027. Keywords: DNA methylation, S5 classifier, Human papillomavirus, EPB41L3, Triage, Cervical intraepithelial neoplasia, Cervical cancer