학술논문
Metformin exerts anti-inflammatory effects on mouse colon smooth muscle cells in vitro
Document Type
Report
Source
Experimental and Therapeutic Medicine. August 2018, Vol. 16 Issue 2, p985, 8 p.
Subject
Language
English
ISSN
1792-0981
Abstract
Introduction Mounting evidence suggests that intestinal smooth muscle cells (SMCs) may be involved in different inflammatory diseases that affect the bowel, leading to altered morphology, contractility and augmented production of [...]
Inflammatory bowel disease (IBD) is a chronic incurable condition characterized by relapsing inflammation of the gut. Intestinal smooth muscle cells (SMCs) are affected structurally and functionally during IBD due to excessive production of different inflammatory mediators. Metformin is a widely used antidiabetic agent known to exert several anti-inflammatory effects in different tissues independently from its hypoglycemic effect. The aim of the present study was to investigate the effect of metformin on expression and secretion of different cytokines and chemokines from mouse colon SMCs (CSMCs) following induction of inflammation with lipopolysaccharide (LPS) in vitro. CSMCs from male BALB/c mice were isolated and cultured in Dulbecco's modified Eagle's medium and treated with LPS (1 [micro]g/ml) and 0, 5, 10 or 20 mM metformin for 24 h. Expression and secretion of tumor necrosis factor-[alpha] (TNF-[alpha]), interleukin-1[alpha] (IL- 1[alpha]), macrophage colony stimulating factor (M-CSF), T cell activation gene-3 (TCA-3) and stromal cell-derived factor-1 (SDF-1) was evaluated by ELISA. LPS-treated CSMCs demonstrated significantly increased expression of TNF-[alpha], IL-1[alpha], M-CSF, TCA-3 and SDF-1 when compared with the control group (P Key words: metformin, inflammation, inflammatory bowel disease, smooth muscle
Inflammatory bowel disease (IBD) is a chronic incurable condition characterized by relapsing inflammation of the gut. Intestinal smooth muscle cells (SMCs) are affected structurally and functionally during IBD due to excessive production of different inflammatory mediators. Metformin is a widely used antidiabetic agent known to exert several anti-inflammatory effects in different tissues independently from its hypoglycemic effect. The aim of the present study was to investigate the effect of metformin on expression and secretion of different cytokines and chemokines from mouse colon SMCs (CSMCs) following induction of inflammation with lipopolysaccharide (LPS) in vitro. CSMCs from male BALB/c mice were isolated and cultured in Dulbecco's modified Eagle's medium and treated with LPS (1 [micro]g/ml) and 0, 5, 10 or 20 mM metformin for 24 h. Expression and secretion of tumor necrosis factor-[alpha] (TNF-[alpha]), interleukin-1[alpha] (IL- 1[alpha]), macrophage colony stimulating factor (M-CSF), T cell activation gene-3 (TCA-3) and stromal cell-derived factor-1 (SDF-1) was evaluated by ELISA. LPS-treated CSMCs demonstrated significantly increased expression of TNF-[alpha], IL-1[alpha], M-CSF, TCA-3 and SDF-1 when compared with the control group (P Key words: metformin, inflammation, inflammatory bowel disease, smooth muscle