학술논문
A generalised module for the selective extracellular accumulation of recombinant proteins
Document Type
Report
Author
Source
Microbial Cell Factories. May 28, 2012, Vol. 11 Issue 1
Subject
Language
English
ISSN
1475-2859
Abstract
Author(s): Yanina R Sevastsyanovich[sup.1] , Denisse L Leyton[sup.1] , Timothy J Wells[sup.1] , Catherine A Wardius[sup.1] , Karina Tveen-Jensen[sup.1] , Faye C Morris[sup.1] , Timothy J Knowles[sup.2] , Adam F [...]
Background It is widely believed that laboratory strains of Escherichia coli, including those used for industrial production of proteins, do not secrete proteins to the extracellular milieu. Results Here, we report the development of a generalised module, based on an E. coli autotransporter secretion system, for the production of extracellular recombinant proteins. We demonstrate that a wide variety of structurally diverse proteins can be secreted as soluble proteins when linked to the autotransporter module. Yields were comparable to those achieved with other bacterial secretion systems. Conclusions The advantage of this module is that it relies on a relatively simple and easily manipulated secretion system, exhibits no apparent limitation to the size of the secreted protein and can deliver proteins to the extracellular environment at levels of purity and yields sufficient for many biotechnological applications. Keywords: Autotransporter, Escherhichia coli, Recombinant protein production, Secretion
Background It is widely believed that laboratory strains of Escherichia coli, including those used for industrial production of proteins, do not secrete proteins to the extracellular milieu. Results Here, we report the development of a generalised module, based on an E. coli autotransporter secretion system, for the production of extracellular recombinant proteins. We demonstrate that a wide variety of structurally diverse proteins can be secreted as soluble proteins when linked to the autotransporter module. Yields were comparable to those achieved with other bacterial secretion systems. Conclusions The advantage of this module is that it relies on a relatively simple and easily manipulated secretion system, exhibits no apparent limitation to the size of the secreted protein and can deliver proteins to the extracellular environment at levels of purity and yields sufficient for many biotechnological applications. Keywords: Autotransporter, Escherhichia coli, Recombinant protein production, Secretion