학술논문
Transcriptional regulation of HIV-1 host factor COMMD1 by the Sp family
copper metabolism Murr1 domain containing 1
copper metabolism Murr1 domain containing 1
Document Type
Report
Source
International Journal of Molecular Medicine. April 2018, Vol. 41 Issue 4, p2366, 9 p.
Subject
Language
English
ISSN
1107-3756
Abstract
Introduction Copper metabolism Murr1 domain containing 1 (COMMD1) is a member of the COMMD family, which has ten members. This protein family is distinguished by a unique C-terminal motif called [...]
Copper metabolism Murr1 domain containing 1 (COMMD1) has multiple functions in the regulation of protein stability at the plasma membrane and in the cytoplasm. However, the regulation of COMMD1 transcriptional has remained to be elucidated. In the present study, the 5'-flanking region (-1,192/+83 bp) of the human COMMD1 gene was cloned. It was observed that the COMMD1 promoter region contains Gc-rich region that has 7 putative Sp1-binding sites via in silico analysis. The proximal promoter region at -289/+83 bp was required for COMMD1 basal promoter activity by deletion constructs of COMMD1 promoter. Moreover, Sp1 inhibitor, mithramycin A, suppressed basal COMMD1 promoter activity. The Sp1-binding site bp) in the proximal promoter region was a critical site for COMMD1 gene regulation by Sp1 and Sp3. Sp1 upregulated COMMD1 promoter activity, whereas Sp3 suppressed it. Endogenous Sp1 and Sp3 bound to the proximal promoter region of COMMD1. Taken together, Spl constitutively regulates the basal expression of the COMMD1 gene in human epithelial cell lines. Key words: copper metabolism Murr1 domain containing 1, transcriptional regulation, Sp1
Copper metabolism Murr1 domain containing 1 (COMMD1) has multiple functions in the regulation of protein stability at the plasma membrane and in the cytoplasm. However, the regulation of COMMD1 transcriptional has remained to be elucidated. In the present study, the 5'-flanking region (-1,192/+83 bp) of the human COMMD1 gene was cloned. It was observed that the COMMD1 promoter region contains Gc-rich region that has 7 putative Sp1-binding sites via in silico analysis. The proximal promoter region at -289/+83 bp was required for COMMD1 basal promoter activity by deletion constructs of COMMD1 promoter. Moreover, Sp1 inhibitor, mithramycin A, suppressed basal COMMD1 promoter activity. The Sp1-binding site bp) in the proximal promoter region was a critical site for COMMD1 gene regulation by Sp1 and Sp3. Sp1 upregulated COMMD1 promoter activity, whereas Sp3 suppressed it. Endogenous Sp1 and Sp3 bound to the proximal promoter region of COMMD1. Taken together, Spl constitutively regulates the basal expression of the COMMD1 gene in human epithelial cell lines. Key words: copper metabolism Murr1 domain containing 1, transcriptional regulation, Sp1