부산대학교 도서관

Propolis, a resinous substance collected by honeybees by mixing their saliva with plant sources, including tree bark and leaves and then mixed with secreted beeswax, possesses a variety of bioactivities. Whereas caffeic acid phenethyl ester (CAPE) has been recognized as a major bioactive ingredient in New Zealand propolis, Brazilian green propolis, on the other hand, possesses artepillin C (ARC). In this study, we report that, similar to CAPE, ARC docks into and abrogates mortalin-p53 complexes, causing the activation of p53 and the growth arrest of cancer cells. Cell viability assays using ARC and green propolis-supercritical extract (GPSE) revealed higher cytotoxicity in the latter, supported by nuclear translocation and the activation of p53. Furthermore, in vivo tumor suppression assays using nude mice, we found that GPSE and its conjugate with [gamma] cyclodextrin ([gamma]CD) possessed more potent anticancer activity than purified ARC. GPSE-[gamma]CD may thus be recommended as a natural, effective and economic anti-cancer amalgam. Key words: Brazilian green propolis, supercritical extract, [gamma] cyclo-dextrin conjugate, p53-mortalin complex, abrogation, anticancer
Introduction Propolis is a complex mixture of resinous material, produced by bees, created by mixing their saliva with the botanical sources they live on. The color (yellowish green to dark [...]