부산대학교 도서관

The article describes the investigation of OFFGEL fractionation of mouse kidney protein lysate and its tryptic peptide digest that was carried out to better understand the differences between protein and peptide fractionation methods and attaining maximum recruitment of this modern methodology for in-depth proteomic analysis. OFFGEL fractionation favored protein identification, fractionation efficiency, and focusing resolution, while peptide OFFGEL was better in recovery, number of peptide matches, and protein coverage. This result suggested that the protein fractionation method was more suitable for shotgun analysis while peptide fractionation was suitable for quantitative peptide analysis [isobaric tags for relative and absolute quantitation (iTRAQ) or tandem mass tags (TMT)].