학술논문
Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach
Document Type
Report
Author
Source
Analytical and Bioanalytical Chemistry. September 1, 2015, p7157, 7 p.
Subject
Language
English
ISSN
1618-2642
Abstract
Author(s): Rita C. Alves[sup.1] [sup.2] , Filipa B. Pimentel[sup.2] , Henri P. A. Nouws[sup.1] , Wagner Correr[sup.3] , Maria Begoña Gonzalez-Garcia[sup.1] , M. Beatriz P. P. Oliveira[sup.2] , Cristina Delerue-Matos[sup.1] [...]
A voltammetric biosensor for Ara h 6 (a peanut allergen) detection in food samples was developed. Gold nanoparticle-modified screen-printed carbon electrodes were used to develop a sandwich-type immunoassay using two-monoclonal antibodies. The antibody-antigen interaction was detected through the electrochemical detection of enzymatically deposited silver. The immunosensor presented a linear range between 1 and 100 ng/ml, as well as high precision (inter-day RSD ≤9.8 %) and accuracy (recoveries ≥96.7 %). The detection and quantification limits were 0.27 and 0.88 ng/ml, respectively. It was possible to detect small levels of Ara h 6 in complex food matrices.
A voltammetric biosensor for Ara h 6 (a peanut allergen) detection in food samples was developed. Gold nanoparticle-modified screen-printed carbon electrodes were used to develop a sandwich-type immunoassay using two-monoclonal antibodies. The antibody-antigen interaction was detected through the electrochemical detection of enzymatically deposited silver. The immunosensor presented a linear range between 1 and 100 ng/ml, as well as high precision (inter-day RSD ≤9.8 %) and accuracy (recoveries ≥96.7 %). The detection and quantification limits were 0.27 and 0.88 ng/ml, respectively. It was possible to detect small levels of Ara h 6 in complex food matrices.