학술논문
Galactaro delta-lactone isomerase: lactone isomerization by a member of the amidohydrolase superfamily
Document Type
Report
Author
Source
Biochemistry. Feb 4, 2014, Vol. 53 Issue 4, p614
Subject
Language
English
ISSN
0006-2960
Abstract
The article describes a novel enzyme, d-galactarolactone isomerase (GLI), that catalyzes the isomerizaton of d-galactaro-1,5-lactone to d-galactaro-1,4-lactone in Agrobacterium tumefaciens strain C58. This GLI is the member of the functionally diverse amidohydrolase superfamily and is a homologue of LigI that catalyzes the hydrolysis of 2-pyrone-4,6-dicarboxylate in lignin degradation. Furthermore, the absence of the general basic catalysis used by 2-pyrone-4,6-dicarboxylate lactonase can explain the ability of GLI to catalyze lactone isomerization instead of hydrolysis.