부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.aca.2009.05.022 Byline: Bruno Lemos Batista, Denise Grotto, Jairo Lisboa Rodrigues, Vanessa Cristina de Oliveira Souza, Fernando Barbosa Keywords: Sample preparation; Biological tissues; Trace elements; TMAH; DRC-ICP-MS Abstract: A simple method for sample preparation of biological samples for trace elements determination by inductively coupled plasma mass spectrometry (ICP-MS) is described. Prior to analysis, 75mg of the biological samples were accurately weighed into (15mL) conical tubes. Then, 1mL of 50% (v/v) tetramethylammonium hydroxide (TMAH) solution was added to the samples, incubated at room temperature for 12h and the volume made up to 10mL with a solution containing 0.5% (v/v) HNO.sub.3, 0.01% (v/v) Triton.sup.[R] X-100 and 10[mu]gL.sup.-1 of Rh. After preparation samples may be stored at -20[degrees]C during 3 days until the analysis by ICP-MS. With these conditions, the use of the dynamic reaction cell was only mandatory for chromium determination. Method detection limits were 0.2145, 0.0020, 0.0051, 0.0017, 0.0027, 0.0189, 0.02, 0.5, 0.1, 0.0030, 0.0043, 0.0066, 0.0009, 0.020, 0.0043, 0.1794, 0.1[mu]gg.sup.-1 for Al, As, Ba, Cd, Co, Cr, Cu, Fe, Mg, Mn, Mo, Pb, Sb, Se, Sr, V and Zn, respectively. Validation data are provided based on the analysis of six certified reference materials (CRMs) purchased from the National Institute of Standards and Technology (NIST) and National Research Council Canada (NRCC). Additional validation was provided by the analysis of brain, kidney, liver and heart samples collected from rats and analyzed by the proposed method and by using microwave digestion. Author Affiliation: Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de CiA*ncias FarmacA*uticas de Ribeirao Preto, FCFRP, USP, Avenida do Cafe, s/n, Monte Alegre, 14040-903, Ribeirao Preto, SP, Brazil Article History: Received 24 March 2009; Revised 11 May 2009; Accepted 14 May 2009