부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.anireprosci.2006.02.002 Byline: Marcelo Ratto (a), Constanza Gomez (b), Marco Berland (b), Gregg P. Adams (a) Keywords: Alpaca; Gonadotropin superstimulation; Oocyte collection; In vitro maturation Abstract: The objective of the present study was to compare the ovarian follicular response, cumulus-oocyte complex (COC) collection rate, and maturational status of COC collected from alpacas subsequent to treatment with two different superstimulatory protocols. Alpacas (n =7 per group) were treated with: (1) 200mg of FSH im divided bid for 3d, plus a single i.v. dose of 1000IU hCG 24h after the last FSH treatment, or (2) 1200IU of eCG as a single i.m. dose, plus a single i.v. dose of 1000IU of hCG on day 3 after eCG treatment (day 0=start of superstimulatory treatment). At 20-24h post-hCG treatment, the ovaries were surgically exposed and COC were collected by needle aspiration of all follicles [greater than or equal to]6mm. The FSH and eCG treatment groups did not differ with respect to the number of follicles [greater than or equal to]6mm at the time of COC collection (20.0[+ or -]7.5 versus 27.0[+ or -]3.3; P =0.5), the number of COC collected (26.2[+ or -]8.4 versus 23.3[+ or -]3.7; P =0.7), or the collection rate per follicle aspirated (89% versus 87%; P =0.7). No differences were detected between FSH- and eCG-treated alpacas in the number of expanded COC collected per alpaca (11.5[+ or -]2.9 versus 8.8[+ or -]2.8; P =0.54), the number of expanded COC in metaphase II (8.5[+ or -]1.9 versus 6.0[+ or -]2.1; P =0.1), or the number of compact COC with [greater than or equal to]3 layers of cumulus cells (12.5[+ or -]4.3 versus 14.3[+ or -]2.6; P =0.72). A greater proportion (P Author Affiliation: (a) Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada S7N 5B4 (b) Laboratory of Animal Reproduction, Faculty of Veterinary Medicine, Catholic University of Temuco, M. Mont 056, Temuco, Chile Article History: Received 14 July 2005; Accepted 1 February 2006