부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.exppara.2006.04.005 Byline: Jenny Telleria (a), Benedicte Lafay (a), Myrna Virreira (b), Christian Barnabe (a), Michel Tibayrenc (a), Michal Svoboda (b) Keywords: Trypanosoma cruzi; Minicircles; Kinetoplast DNA (kDNA) polymorphism; Hypervariable domain; DTU (Discrete Typing Units); Evolution; Editing Abstract: The comparisons of 170 sequences of kinetoplast DNA minicircle hypervariable region obtained from 19 stocks of Trypanosoma cruzi and 2 stocks of Trypanosoma cruzi marenkellei showed that only 56% exhibited a significant homology one with other sequences. These sequences could be grouped into homology classes showing no significant sequence similarity with any other homology group. The 44% remaining sequences thus corresponded to unique sequences in our data set. In the DTU I ("Discrete Typing Units") 51% of the sequences were unique. In contrast, in the DTU IId, 87.5% of sequences were distributed into three classes. The results obtained for T. cruzi marinkellei, showed that all sequences were unique, without any similarity between them and T. cruzi sequences. Analysis of palindromes in all sequence sets show high frequency of the EcoRI site. Analysis of repetitive sequences suggested a common ancestral origin of the kDNA. The editing mechanism that occurs in kinetoplastidae is discussed. Author Affiliation: (a) Laboratoire de Genetique et Evolution des Maladies Infectieuses, UMR CNRS-IRD 2724 and UR IRD 165, Centre IRD, 911 avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France (b) Laboratoire de Chimie Biologique, Faculte de Medecine, Universite Libre de Bruxelles, Route de Lennik 808 CP611, B-1070 Brussels, Belgium Article History: Received 28 February 2006; Revised 11 April 2006; Accepted 11 April 2006