부산대학교 도서관

In the present study we investigated the effects of dietary fats containing predominantly PUFA, monounsaturated FA (MUFA), or saturated FA (SFA) on lipid profile and liver cholesterol 7[alpha]-hydroxylase (CYP7[alpha]1) mRNA expression and bile acid production in C57BL/6J mice. The animals (n=75) were randomly divided into five groups and fed a basic chow diet (AIN-93G) (BC diet), a chow diet with 1g/100g of cholesterol (Chol diet), a chow diet with 1g/100g of cholesterol and 14g/100g of safflower oil (Chol+PUFA diet), a chow diet with 1g/100g of cholesterol and olive oil (Chol+MUFA diet), or a chow diet with 1g/100g of cholesterol and myristic acid (Chol+SFA diet) for 6 wk. The results showed that the Chol+SFA diet decreased CYP7[alpha]1 gene expression and bile acid pool size, resulting in increased blood and liver cholesterol levels. Addition of PUFA and MUFA to a 1% cholesterol diet increased the bile acid pool production or bile acid excretion and simultaneously decreased liver cholesterol accumulation despite decreased CYP7[alpha]1 mRNA expression. The results indicate that the decreased bile acid pool size induced by the SFA diet is related to inhibition of the liver CYP7[alpha]1 gene expression, but an increased bile acid pool size and improved cholesterol homeostasis are disassociated from the liver CYP7[alpha]1 gene expression.