부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.jdiacomp.2006.05.005 Byline: Amy-Jayne McKnight (a), A. Peter Maxwell (a), Chris C. Patterson (b), Hugh R. Brady (c), David A. Savage (a) Keywords: Association; Nephropathy; SNP; Type 1 diabetes; VEGF Abstract: Vascular endothelial growth factor (VEGF) is reported to be implicated in the development of diabetic nephropathy. We performed a case-control study to determine if VEGF-2578C[right arrow]A, VEGF-1499C[right arrow]T, and VEGF-635G[right arrow]C single-nucleotide polymorphisms (SNPs) in the VEGF gene are associated with predisposition to diabetic nephropathy in type 1 diabetes. Genomic DNA was obtained from Irish type 1 diabetic individuals with nephropathy (cases, n=242) and those without nephropathy (controls, n=301), in addition to 400 healthy control samples. These samples were genotyped for the three SNPs using TaqMan or Pyrosequencing technology. Chi-squared analyses revealed no significant differences in genotype or allele frequencies in cases versus controls for VEGF-2578C[right arrow]A (genotype, P=.58; allele, P=.52) and VEGF-635G[right arrow]C (genotype, P=.58; allele, P=.33). However, a positive association with diabetic nephropathy was observed for the VEGF-1499T allele in the Northern Ireland population (P Our results suggest that the VEGF-1499T allele, or an allele in linkage disequilibrium with this allele, is associated with susceptibility to diabetic nephropathy in the Irish population. Author Affiliation: (a) Nephrology Research Group, Queen's University of Belfast, Belfast, UK (b) Department of Epidemiology and Public Health, Queen's University of Belfast, Belfast, UK (c) Dublin Molecular Medicine Centre, Conway Institute, Dublin, Ireland Article History: Received 21 February 2006; Accepted 8 May 2006