부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.anifeedsci.2006.05.010 Byline: Nelson Perez Guerra (a), Paula Fajardo Bernardez (a), Jesus Mendez (b), Pilar Cachaldora (b), Lorenzo Pastrana Castro (a) Keywords: Body weight; Feed intake; Gastrointestinal tract; Lactic acid bacteria; Piglets; Probiotic Abbreviations: BWG, body weight gain; CECT, Spanish Type Culture Collection; CMPW, concentrated mussel-processing wastes medium; CW, concentrated whey medium; CWYE, concentrated whey medium supplemented with a 2% (w/v) yeast extract; DW, diluted whey medium; DWYE, DW medium supplemented with a 2% (w/v) yeast extract; FCE, feed conversion efficiency; FI, feed intake; LAB, lactic acid bacteria; MRS, de Man, Rogosa and Sharpe medium; PBS, phosphate-buffered saline Abstract: The present study was conducted to study the production and evaluation of potentially probiotic additives containing both live lactic acid bacteria (Pediococcus acidilactici NRRL B-5627, Lactococcus lactis subsp. lactis CECT 539, Lactobacillus casei subsp. casei CECT 4043 and Enterococcus faecium CECT 410) and antimicrobial metabolites with could be used as a replacement for antibiotics in weanling pig diets. The gastrointestinal transit tolerance of the four bacteria was determined by exposing washed cell suspensions at 30[degrees]C to acidic conditions (pH 1.0, 2.0, 3.0, 4.0 and 5.0), to a simulated gastric juice (pH 2.0) containing pepsin (3g/L) and sodium chloride (5g/L), and to a simulated small intestinal juice (pH 8.0) containing pancreatin (1g/L) and sodium chloride (5g/L), mimicking the gastrointestinal environment. These studies showed that the four strains are capable of surviving the passage through the gastrointestinal conditions. Therefore, the production of biomass and antimicrobial products by these bacteria was performed in whey using a fed-batch fermentation technique that provided enhanced productions compared to those obtained in batch fermentations. The obtained fed-batch cultures preserved at -20[degrees]C with skim milk showed a good viability after 3 months of storage. The four cultures exhibited low losses of viability in the piglet feed during their storage at room temperature for 8 days. These results offered the possibility of using the piglet feed as a vehicle to administer the four probiotic bacteria. The effects of the supplementation of separate potentially probiotic cultures and an antibiotic (colistin sulfate) to piglet diets on body weight gain, feed intake, feed efficiency and on the faecal coliform counts of weaned piglets were also studied. Although the best results were obtained in the groups receiving the antibiotic, a significant increase in body weight gain and final body weight was obtained in the groups fed diets supplemented with lactic acid bacteria as compared with the non-treated (controls) groups (P=0.05). The changes in the total coliform population in the control groups over time were not significant (P Author Affiliation: (a) Departamento de Bioquimica, Xenetica e Inmunoloxia, Facultade de Ciencias de Ourense, Universidade de Vigo, As Lagoas s/n, 32004 Ourense, Spain (b) Cooperativas Orensanas Sociedad Cooperativa Ltda (COREN), Poligono San Ciprian de ViA[+ or -]as, 32901 Ourense, Spain Article History: Received 26 January 2006; Revised 14 April 2006; Accepted 8 May 2006