부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.pestbp.2006.09.001 Byline: Jian-Rong Gao, Toshinori Kozaki, Cheryl A. Leichter, Frank D. Rinkevich, Toshio Shono, Jeffrey G. Scott Keywords: Insecta; Musca domestica; Population genetics; Insecticide resistance; Fitness disadvantage Abstract: Fipronil is a relatively new insecticide with great potential for insect control, however widespread use of cyclodiene insecticides has selected for an A302S mutation in the Rdl (GABA gated chloride channel) allele. This mutation gives resistance to cyclodienes and limited cross-resistance to fipronil. Given the concern over the possible reduction in efficacy and/or lifetime that fipronil might be used for pest control (given the extensive use of cyclodienes in the past), it is important to know the frequency of the A302S Rdl mutation in field populations. To ascertain the relative frequency of the A302S Rdl mutation in house fly populations we used three experimental approaches. First, we attempted to select for fipronil resistance by initially treating 33,100 field collected flies and selecting 14 additional generations. We were unable to produce a highly resistant strain. Second, we directly sequenced field collected flies. Third, we tested field collected house flies with a diagnostic dose of dieldrin and then genotyped the survivors. Out of the 4750 flies tested, there were no Rdl resistance alleles detected. We conclude that the resistant Rdl allele is rare in house flies in the US due to decades without cyclodiene use and a fitness disadvantage (in the absence of cyclodienes) of the 302S Rdl allele. The limited cross-resistance provided by the cyclodiene resistant Rdl allele, combined with the very low frequency of this allele in field populations, suggests that fipronil could be a promising insecticide for house fly control. Author Affiliation: Department of Entomology, Comstock Hall, Cornell University, Ithaca, NY 14853-0901, USA Article History: Received 2 August 2006; Accepted 12 September 2006