학술논문
HPV-16-related DNA sequences in Kaposi's sarcoma
Document Type
Academic Journal
Author
Source
The Lancet. Feb 29, 1992, Vol. 339 Issue 8792, p515, 4 p.
Subject
Language
ISSN
0140-6736
Abstract
A sarcoma is a tumor, often malignant, composed of cells similar to embryonic connective tissue. Among patients with AIDS, Kaposi's sarcoma is ten times more common in bisexual or homosexual than heterosexual men. Because of the high incidence of human papillomavirus (HPV) in homosexual men, a link was sought between Kaposi's sarcoma and HPV-16. HPV-16 DNA fragments were found in 11 of 69 tumors from homosexual men with AIDS, in 5 of 17 tumors from men and women who tested HIV negative, and in 3 of 11 homosexual men who had no evidence of HIV infection. HPV-16 may be associated with some forms of Kaposi's sarcoma.
In the USA, Kaposi's sarcoma associated with the acquired immunodeficiency syndrome (AIDS-KS) is ten times more common in homosexual or bisexual men than in heterosexual men with AIDS. One explanation for this finding is that AIDS-KS may be caused by an infectious agent. Because there is a high incidence of human papillomavirus (HPV) infection, especially HPV-16, in homosexual men, we have sought HPV DNA sequences in Kaposi's sarcoma. We used the polymerase chain reaction with a primer pair specific for the highly conserved E6 region of HPV-16 to detect HPV-16 homologous DNA fragments in tumour tissues from 97 patients with KS and in KS-derived cell cultures. HPV DNA sequences were found in 11 of 69 KS skin tumours from homoexsual men with AIDS-KS, in 3 of 11 KS biopsy specimens from homosexual men who had no clinical or laboratory evidence of HIV-infection, and in 5 of 17 KS skin lsions from HIV-1-negative elderly men and women with classic KS. The same primer pair amplified HPV-16 homologous fragments from two different continuous cell cultures derived from pleural effusion fluid of patients with pulmonary AIDS-KS and two continuous cell cultures derived from KS skin lesions. The findings suggest the HPV-16-related DNA sequences are associated with different forms of KS and may have a role in the pathogenesis of this neoplasm.
In the USA, Kaposi's sarcoma associated with the acquired immunodeficiency syndrome (AIDS-KS) is ten times more common in homosexual or bisexual men than in heterosexual men with AIDS. One explanation for this finding is that AIDS-KS may be caused by an infectious agent. Because there is a high incidence of human papillomavirus (HPV) infection, especially HPV-16, in homosexual men, we have sought HPV DNA sequences in Kaposi's sarcoma. We used the polymerase chain reaction with a primer pair specific for the highly conserved E6 region of HPV-16 to detect HPV-16 homologous DNA fragments in tumour tissues from 97 patients with KS and in KS-derived cell cultures. HPV DNA sequences were found in 11 of 69 KS skin tumours from homoexsual men with AIDS-KS, in 3 of 11 KS biopsy specimens from homosexual men who had no clinical or laboratory evidence of HIV-infection, and in 5 of 17 KS skin lsions from HIV-1-negative elderly men and women with classic KS. The same primer pair amplified HPV-16 homologous fragments from two different continuous cell cultures derived from pleural effusion fluid of patients with pulmonary AIDS-KS and two continuous cell cultures derived from KS skin lesions. The findings suggest the HPV-16-related DNA sequences are associated with different forms of KS and may have a role in the pathogenesis of this neoplasm.