부산대학교 도서관

We present here work towards the development of a lab-on-a-chip device used in the quantification of secreted cytokines from a small population of captured immune cells. This is accomplished by integrating microfluidic technology with interdigitated microelectrodes, which are used to quantify proteins using electrochemical impedance spectroscopy. Presented in detail is the effect of several parameters on the performance of the electrochemical biosensors, and a brief discussion of the microfluidic technology integrated with these biosensors in order to create a platform for cellular immunophenotyping. The developed sensor had a dynamic range from 0.1 ng/mL to 100 ng/mL, and the detection sensitivities at various frequencies are noted. It was also found that a redox probe concentration of 2mM is optimum for EIS measurements.