학술논문
Shape Anisotropic Magnetic Particles for high throughput and high efficiecy intracelluar delivery of functional macromolecules
Document Type
Conference
Author
Source
2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS) Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on. :880-883 Jun, 2015
Subject
Language
ISSN
2159-547X
2164-1641
2164-1641
Abstract
We report a novel intracellular delivery system based on aligning Shape-Anisotropic Magnetic Particles (SAMP) to deliver functional molecules into mammalian cells with high efficiency and high viability. A broad range of molecules (calcein, dextran 3K, dextran 40K, GFP plasmid, SiRNA, and β-lactamase) have been successfully delivered into primary cells with preserved functions. We have achieved high efficiency delivery of enzyme into primary human dermal fibroblast (NHDF), and other large macromolecules (dextran 40K) into mouse cortical neurons.