부산대학교 도서관

Bioelectronic devices for the investigation of electrogenic cells either comprise advanced system architectures (K. Gilchrist et al., Biosens. & Bioelects., vol. 16, pp. 557-564, 2001) or patterned structures for guided cell growth (S. Rohr et al., Pflugers Arch., vol. 446, pp. 125-32, 2003). Here we present an approach to combine a CMOS biosensor with sophisticated surface patterning for guided cell growth. The CMOS biosensor comprises a 128-electrode array, with on-chip stimulation and recording capabilities. An engineered surface for high-contrast protein adsorption and cell attachment has been developed. Surface functionalization is based on selective molecular assembly patterning (SMAP) (R. Michel et al., Langmuir, vol. 18, pp. 3281-3287, 2002) thus obviating the need for additional lithographic steps. An amine-terminated self-assembled monolayer is used for cell adhesion at each electrode site. A protein-repellent grafted copolymer, poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG), is used to render the surrounding silicon oxide resistant to protein adsorption.