학술논문

Image Denoising in Femtosecond Stimulated Raman Scattering Microscopy
Document Type
Conference
Source
2023 23rd International Conference on Transparent Optical Networks (ICTON) Transparent Optical Networks (ICTON), 2023 23rd International Conference on. :1-4 Jul, 2023
Subject
Communication, Networking and Broadcast Technologies
Components, Circuits, Devices and Systems
Engineered Materials, Dielectrics and Plasmas
Fields, Waves and Electromagnetics
Photonics and Electrooptics
Signal Processing and Analysis
Transportation
Optical microscopy
Microscopy
Raman scattering
Noise reduction
Transforms
Lipidomics
Throughput
nonlinear microscopy
label-free imaging
stimulated Raman scattering
noise
image denoising
Language
ISSN
2161-2064
Abstract
Stimulated Raman scattering (SRS) microscopy is able to perform label-free imaging of lipids with high sensitivity, high spatial and spectral resolution, 3D sectioning, and fast time of image acquisition, i.e., a few seconds. Nevertheless, due to the weak Raman cross-section of biomolecules, SRS images often suffer from low SNR, so SRS applications in biological/biomedical imaging can be compromised. Therefore, methods aiming to improve image quality are required. In this paper, we compare the performances of two widespread general algorithms, i.e. Singular Value Decomposition (SVD) and Discrete Wavelet Transform (DWT). In order to validate our algorithms, in our investigations lipids droplets (LDs) have been used.