학술논문
Evolution of Plasmodium falciparum antimalarial drug resistance markers post-adoption of artemisinin-based combination therapies in Yaounde, Cameroon
Document Type
article
Author
Peter Thelma Ngwa Niba; Akindeh Mbuh Nji; Jean Paul Kengne Chedjou; Helle Hansson; Emma Filtenborg Hocke; Innocent Mbulli Ali; Olivia Achonduh-Atijegbe; Marie-Solange B. Evehe; Marie Helene Munck Jørgensen; Calvino Tah Fomboh; Liwang Cui; Gillian Stresman; Jude D. Bigoga; Michael Alifrangis; Wilfred F. Mbacham
Source
International Journal of Infectious Diseases, Vol 132, Iss , Pp 108-117 (2023)
Subject
Language
English
ISSN
1201-9712
18849881
18849881
Abstract
Objectives: This study aimed to investigate the evolution of Plasmodium falciparum antimalarial drug resistance markers by comparing the pre- and post-adoption of artemisinin-based combination therapies (ACTs) in Yaounde, Cameroon. Methods: The molecular characterization of known antimalarial drug resistance markers (Pfcrt, Pfmdr1, Pfdhfr, Pfdhps, and Pfk13) in P. falciparum-positive samples collected in 2014 and 2019-2020 was achieved using nested polymerase chain reaction, followed by targeted amplicon deep sequencing on the Illumina MiSeq platform. Data derived were compared with those published during the pre-ACT adoption period from 2004 to 2006. Results: A high prevalence of Pfmdr1 184F, Pfdhfr 51I/59R/108N, and Pfdhps 437G mutant alleles was observed during the post-ACT adoption period. The Pfcrt 76T and Pfmdr1 86Y mutant alleles significantly declined between 2004 and 2020 (P