학술논문

Impact du séchage sur la viabilité de Pseudomonas fluorescens (synthèse bibliographique)
Document Type
article
Source
Biotechnologie, Agronomie, Société et Environnement, Vol 18, Iss 1, Pp 134-141 (2014)
Subject
Pseudomonas fluorescens
fatty acids
cryoprotectants
freeze-drying
viability
Biotechnology
TP248.13-248.65
Environmental sciences
GE1-350
Language
English
French
ISSN
1370-6233
1780-4507
Abstract
Impact of drying on Pseudomonas fluorescens viability. A review. Drying Pseudomonas fluorescens makes for more economical storage, transportation and marketing. The aim of the drying process is to stop and to stabilize all biological activity for optimal storage, compatible with the conservation of the maximum desired viability of the microorganisms. However, the viability rate of the bacteria after drying depends on the operating conditions of the drying process. One of the most important criteria to consider during the drying of biologically active products is the quality of the final dried product. Freeze-drying is the drying method most commonly used for Pseudomonas fluorescens. After their production, the bacteria are harvested by centrifugation and are freeze-dried, but the changes in temperature induced by freeze-drying are not without consequence for the cells. The freeze-drying process induces cell damage: peroxidation of fatty acids and proteins and DNA oxidation. However, use of protective compounds during freeze-drying and during storage increases significantly the rate of cell viability.