부산대학교 도서관

Multifunctionality of microalgae is becoming increasingly important, hence science develops new techniques to maximize their potential by providing food, sustainable and affordable fuels and innovative environmental solutions. In this study, we analyzed the effect of different nutrient sources (Nitrofoska®, Quimifol® and Guillard) and sowing time on the kinetics of growth and pigment production of freshwater microalgae Hyaloraphidium contortum; besides of registering some physical and chemical variables in different growth mediums. Bioassays were performed in batch cultures by quadruplicate, continously maintaining and controlling temperature, ventilation and lighting. Growth was determined by cell count and production of pigments by spectrophotometry. The largest population densities and productivities per volume of culture were obtained in F/2 Guillard (9.7±0.2x107 cel mL-1 and 7.6x108 cel/L/ day) and Nitrofoska® (8.7±0.5x107 cel mL-1 and 5.7x108 cel/L/day). The highest average chlorophyll a, chlorophyll b and total carotenoid concentration was achieved with foliar fertilizer Nitrofoska®, on days 18 and 24 (8, 3.29 and 2.2 μg mL-1, respectively), followed by the obtained by Guillard and Quimifol®. We conclude that this microalgae can be grown with commercial agricultural fertilizers as an alternative source of nutrients to produce biomass and pigments with applications in biotechnology and aquaculture industries.