학술논문
A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew
Document Type
article
Author
Miaomiao Li; Huaizhi Zhang; Huixin Xiao; Keyu Zhu; Wenqi Shi; Dong Zhang; Yong Wang; Lijun Yang; Qiuhong Wu; Jingzhong Xie; Yongxing Chen; Dan Qiu; Guanghao Guo; Ping Lu; Beibei Li; Lei Dong; Wenling Li; Xuejia Cui; Lingchuan Li; Xiubin Tian; Chengguo Yuan; Yiwen Li; Dazhao Yu; Eviatar Nevo; Tzion Fahima; Hongjie Li; Lingli Dong; Yusheng Zhao; Zhiyong Liu
Source
Nature Communications, Vol 15, Iss 1, Pp 1-14 (2024)
Subject
Language
English
ISSN
2041-1723
Abstract
Abstract Crop wild relatives offer natural variations of disease resistance for crop improvement. Here, we report the isolation of broad-spectrum powdery mildew resistance gene Pm36, originated from wild emmer wheat, that encodes a tandem kinase with a transmembrane domain (WTK7-TM) through the combination of map-based cloning, PacBio SMRT long-read genome sequencing, mutagenesis, and transformation. Mutagenesis assay reveals that the two kinase domains and the transmembrane domain of WTK7-TM are critical for the powdery mildew resistance function. Consistently, in vitro phosphorylation assay shows that two kinase domains are indispensable for the kinase activity of WTK7-TM. Haplotype analysis uncovers that Pm36 is an orphan gene only present in a few wild emmer wheat, indicating its single ancient origin and potential contribution to the current wheat gene pool. Overall, our findings not only provide a powdery mildew resistance gene with great potential in wheat breeding but also sheds light into the mechanism underlying broad-spectrum resistance.