학술논문
Pediococcus acidilactici CCFM1344 alleviates chronic stress-induced depression by mitigating Gut microbiota imbalance and Neuroinflammation
Document Type
Article
Author
Source
In Food Bioscience January 2025 63
Subject
Language
ISSN
2212-4292
Abstract
Pediococcus acidilactici has demonstrated promising potential as a psychobiotic with positive effects on gut health and depressive behaviors. Prior research has found that the relative levels of lactate in the intestines of both depressed patients and mice are different from normal levels. In this study, we established a chronic unpredictable mild stress mouse model to compare the effects of high-lactate-producing P. acidilactici strain CCFM1344 and low-lactate-producing strain FSDLZ42M3 on depressive symptoms. Our experimental results showed that after four weeks of intervention, CCFM1344 significantly alleviated anxiety-like and depression-like behaviors induced by CUMS. Furthermore, CCFM1344 restored serum LPS levels, as well as the abnormally elevated LPS receptor protein and downstream pro-inflammatory cytokine genes expression in the hippocampus. Conversely, FSDLZ42M3 did not exhibit these effects. Notably, CCFM1344 was found to inhibit the abnormal activation of microglia for the first time. Additionally, 16S rDNA V3-V4 sequencing and untargeted metabolomics assays revealed that CCFM1344 intervention improved gut microbiota dysbiosis. Specifically, it increased the relative abundance of the Lachnospiraceae NK4A136 group and regulated the aberrant intestinal metabolites in mice. These findings expand the potential applications of P. acidilactici as a probiotic and provide theoretical guidance for using CCFM1344 to prevent, alleviate, and treat neurological dysfunction.