학술논문
Non-invasive measurement of cerebral oxygen metabolism in the mouse brain by ultra-high field 17 O MR spectroscopy.
Document Type
Article
Author
Source
Subject
*NONINVASIVE diagnostic tests
*OXYGEN metabolism
*MAGNETIC resonance imaging
*BRAIN physiology
*LABORATORY mice
BRAIN metabolism
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Language
ISSN
0271-678X
Abstract
To assess cerebral energetics in transgenic mouse models of neurologic disease, a robust, efficient, and practical method for quantification of cerebral oxygen consumption is needed. 17O magnetic resonance spectroscopy (MRS) has been validated to measure cerebral metabolic rate of oxygen (CMRO2) in the rat brain; however, mice present unique challenges because of their small size. We show that CMRO2 measurements with 17O MRS in the mouse brain are highly reproducible using 16.4 Tesla and a newly designed oxygen delivery system. The method can be utilized to measure mitochondrial function in mice quickly and repeatedly, without oral intubation, and has numerous potential applications to study cerebral energetics. [ABSTRACT FROM AUTHOR]