학술논문
In vitro infection of primate PBMC with simian/human immunodeficiency virus, SHIV [SUBSF33A]: Correlation to in vivo outcome.
Document Type
Article
Author
Source
Subject
*MACAQUES
*HIV
*
Language
ISSN
0047-2565
Abstract
The macaque/SIV animal system is an important model for studying AIDS pathogenesis and for evaluating the efficacy of vaccines and anti-viral therapeutics. However, differences between HIV-1 and SIV envelope proteins exist that render the SIV/macaque model of limited value when examining envelope determinants of retroviral pathogenesis. To overcome this problem, we utilized a chimeric virus, SHIV[SUBSF33], containing the env gene from HIV-1[SUBSF33] in the context of the molecular clone SIV[SUBmac239], in the macaque animal model. In this study SHIV[SUBSF33A], a pathogenic virus that evolved in vivo from a rhesus macaque infected intravenously with the molecular clone SHIV[SUBSF33] was used in both in vitro and in vivo studies. By using a cell culture system, we examined the biological properties Of our parental and animal-adapted chimeric viruses and compared in vitro susceptibility to in vivo studies. [ABSTRACT FROM AUTHOR]