부산대학교 도서관

A simple technique based on doping polymers with in situ generated silver nanoparticles (Ag/PS films) has been developed. In particular, an antiseptic material has been prepared by dissolving silver 1,5-cyclooctadiene-hexafluoroacetylacetonate in amorphous polystyrene, and the obtained solid solution has been heated for ca. 10 s at a convenient temperature (180°C). Under such conditions the metal precursor decomposes producing silver atoms that diffuse into the polymer and clusterize. The antimicrobial characteristics of the resulting polystyrene-based material have been accurately evaluated toward Escherichia coli (E. coli) comparing the cytotoxicity effect of 10wt.% and 30wt.% (drastic and mild annealing) silver-doped polystyrene to the corresponding puremicrometric silver powder. Two different bacterial viability assays were performed in order to demonstrate the cytotoxic effect of Ag/PS films on cultured E. coli: (1) turbidimetric determination of optical density; (2) BacLight fluorescencebased test. Both methods have shown that silver-doped polystyrene (30wt.%) provides higher antibacterial activity than pure Ag powder, under similar concentration and incubation conditions. [ABSTRACT FROM AUTHOR]