학술논문
Antibody against Porcine Parvovirus VP2 Protein Differentiated This Virus from Other Viruses.
Document Type
Article
Author
Source
Subject
*SWINE physiology
*PARVOVIRUSES
*VIRAL proteins
*IMMUNOGLOBULINS
*GENE expression
*PROKARYOTES
*ESCHERICHIA coli
*POLYACRYLAMIDE gel electrophoresis
*ENZYME-linked immunosorbent assay
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Language
ISSN
0253-8318
Abstract
Viral protein 2 (VP2) of porcine parvovirus (PPV) is the major viral structural protein and responsible for eliciting neutralizing antibodies in immunized animals. In this study, the gene encoding VP2 of PPV was amplified by PCR. The VP2 gene was then cloned into the prokaryotic expression vector, pET-32a followed by expression in Escherichia coli Rosetta. The VP2 protein expression was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Rabbit polyclonal antiserum was generated using the recombinant VP2 protein. The optimal titer of the anti-VP2 antibody was determined by ELISA. The anti-VP2 antibody was able to distinguish PPV from other viruses in ELISA. [ABSTRACT FROM AUTHOR]