학술논문
Modelling real-time simultaneous saccharification and fermentation of lignocellulosic biomass and organic acid accumulation using dielectric spectroscopy
Document Type
Article
Author
Source
Subject
*LIGNOCELLULOSE
*BIOMASS
*FERMENTATION
*ORGANIC acids
*BIOACCUMULATION
*IMPEDANCE spectroscopy
*HYDROLYSIS
*PERENNIALS
*RYEGRASSES
*
*
*
*
*
*
*
*
Language
ISSN
0960-8524
Abstract
Abstract: Dielectric spectroscopy (DS) is routinely used in yeast and mammalian fermentations to quantitatively monitor viable biomass through the inherent capacitance of live cells; however, the use of DS to monitor the enzymatic break down of lignocellulosic biomass has not been reported. The aim of the current study was to examine the application of DS in monitoring the enzymatic saccharification of high sugar perennial ryegrass (HS-PRG) fibre and to relate the data to changes in chemical composition. DS was capable of both monitoring the on-line decrease in PRG fibre capacitance (C =580kHz) during enzymatic hydrolysis, together with the subsequent increase in conductivity (G =580kHz) resulting from the production of organic acids during microbial growth. Analysis of the fibre fractions revealed >50% of HS-PRG lignocellulose had undergone enzymatic hydrolysis. These data demonstrated the utility of DS biomass probes for on-line monitoring of simultaneous saccharification and fermentation (SSF). [Copyright &y& Elsevier]