학술논문
Enhancement of specific cellular immune response induced by DNA vaccines encoding PML-RARα and hIL-2 genes.
Document Type
Article
Author
Source
Subject
*DNA vaccines
*LEUKEMIA
*IMMUNE response
*T-cell receptor genes
*LABORATORY mice
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Language
ISSN
1024-5332
Abstract
A DNA vaccine encoding PML-RAR α fusion gene is thought to be a promising approach for acute promyelocytic leukemia patients to enhance immune responses after attaining complete remission. In this study, we sought to enhance cellular immunity by coexpressing human interleukin (hIL)-2 genes. Successfully constructed plasmids PML-RAR α-hIL-2-pIRES, PML-RAR α-pIRES and hIL-2-pIRES were delivered intramuscularly in BALB/C mice at 14-day intervals for three cycles. The cellular immune responses with respect to the specific cytotoxicity of spleen cells; interferon-γ secretion in sera, and the T-cell receptor rearrangement excision circles of thymocyte were significantly increased from PML-RARα-hIL-2-pIRES immunized mice. Our results indicate that a DNA vaccine with PML fusion gene segment and hIL-2 together might elicit increased cellular immune responses in mice. [ABSTRACT FROM AUTHOR]