학술논문
Genomic characterization of a novel human papillomavirus (HPV-117) with a high viral load in a persisting wart
Document Type
Article
Author
Source
Subject
*PAPILLOMAVIRUSES
*VIRAL genomes
*VIRAL load
*WARTS
*COMPLICATIONS from organ transplantation
*PHYLOGENY
*IMMUNOSUPPRESSION
*MELANOMA
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Language
ISSN
0042-6822
Abstract
Abstract: Warts from immunosuppressed organ transplant recipients (OTR) persist over years and may progress into non-melanoma skin cancer. Human papillomaviruses (HPV) are considered the causal agents for the development of such warts. We isolated the novel type HPV-117 from a persisting wart by rolling circle amplification. One hundred eighteen warts from immunocompetent patients (IC) and 49 warts from OTR were analyzed by HPV-117 E6 type-specific PCR. As inferred from a phylogenetic analysis, the new type HPV-117 belonged to alpha-PV species 2, including the most similar types HPV-10 and HPV-94. The general prevalence of HPV-117 in warts was 2% in IC (2/118), and 12% in OTR (6/49). The high viral load in dysplastic cells of a Verruca vulgaris was shown by in situ hybridization. Our results suggest an active role of the novel type in the development of cutaneous warts of OTR. [Copyright &y& Elsevier]