학술논문
Leptospirosis outbreak in Ecuador in 2023: A pilot study for surveillance from a One Health perspective.
Document Type
Academic Journal
Author
Orlando SA; Instituto Nacional de Salud Pública e Investigación, Guayaquil, Ecuador.; Universidad Espíritu Santo, Guayaquil, Ecuador.; Mora-Jaramillo N; Instituto Nacional de Salud Pública e Investigación, Guayaquil, Ecuador.; Paredes-Núñez D; One Health Research Group, Universidad de Las Américas, Quito, Ecuador.; Rodriguez-Pazmiño AS; One Health Research Group, Universidad de Las Américas, Quito, Ecuador.; Carvajal E; One Health Research Group, Universidad de Las Américas, Quito, Ecuador.; León Sosa A; Instituto Nacional de Salud Pública e Investigación, Guayaquil, Ecuador.; Rivera A; Instituto Nacional de Salud Pública e Investigación, Guayaquil, Ecuador.; Calderon J; Instituto Nacional de Salud Pública e Investigación, Guayaquil, Ecuador.; Guizado Herrera D; Instituto Nacional de Salud Pública e Investigación, Guayaquil, Ecuador.; Arcos F; Universidad Agraria del Ecuador, Guayaquil, Ecuador.; Vera Loor LE; Escuela Superior Politécnica Agropecuaria de Manabí, Calceta, Manabí, Ecuador.; Pérez Oyarvide EV; Ministerio de Salud Pública, Ecuador.; Quimí López DI; Ministerio de Salud Pública, Ecuador.; Guailla Ríos B; Ministerio de Salud Pública, Ecuador.; Benavides Yánez B; Ministerio de Salud Pública, Ecuador.; Torres-Lasso P; Universidad de Guayaquil, Guayaquil, Ecuador.; Zambrano Gavilanes P; Universidad Técnica de Manabí, Portoviejo, Ecuador.; Oviedo MC; Universidad Técnica de Manabí, Portoviejo, Ecuador.; González M; Universidad Ecotec, Guayaquil, Ecuador.; Jiménez-Valenzuela F; Universidad Católica Santiago de Guayaquil, Guayaquil, Ecuador.; Morales García ME; Ministerio de Salud Pública, Ecuador.; Intriago Alcivar EG; Ministerio de Salud Pública, Ecuador.; Saltos Montes JL; Ministerio de Salud Pública, Ecuador.; Medina A; Ministerio de Salud Pública, Ecuador.; López Rauschemberg MK; Escuela Superior Politécnica Agropecuaria de Manabí, Calceta, Manabí, Ecuador.; Zevallos JC; Universidad Espíritu Santo, Guayaquil, Ecuador.; Garcia-Bereguiain MA; One Health Research Group, Universidad de Las Américas, Quito, Ecuador.
Source
Publisher: Elsevier B.V Country of Publication: Netherlands NLM ID: 101660501 Publication Model: eCollection Cited Medium: Print ISSN: 2352-7714 (Print) Linking ISSN: 23527714 NLM ISO Abbreviation: One Health Subsets: PubMed not MEDLINE
Subject
Language
English
ISSN
2352-7714
Abstract
Leptospirosis is a neglected zoonotic disease that is endemic in tropical regions, including Ecuador. It is caused by spirochetes of the genus Leptospira , which can infect humans through animal reservoirs such as rats and dogs, or through contact with contaminated water or soil. In March 2023, public health authorities declared a concerning outbreak of leptospirosis in Durán Cantón, located in the Coastal region of Ecuador. For the first time in the country, a multidisciplinary approach involving physicians and veterinarians was implemented for the surveillance and management of this leptospirosis outbreak. A total of 335 samples were collected, including suspected human cases, household contacts, household dogs, synanthropic rats, and water samples within the area of human cases. Samples were processed by qPCR targeting lipL32 , secY, and rrs fragment genes and characterized further for Sanger sequencing. Overall, 26.2 % of human samples, 43.8 % of dog samples, 38.5 % of rat samples, and 39.4 % of water samples tested positive for Leptospira . Further, phylogenetic analysis shows that human, dog, and rat sequences are clustered within the pathogenic subclade P1, within the branch of L. kirschneri and L. interrogans . This study is the first of its kind in Ecuador, where an ongoing outbreak of leptospirosis was managed in real-time by using molecular diagnosis and not serological tools, and where the epidemiological surveillance was done following a One Health approach. This experience should inspire public and animal health authorities in Ecuador to promote a national One Health surveillance and control program for zoonotic diseases.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2024 The Authors.)
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2024 The Authors.)