학술논문
First report of Bartonella henselae and Bartonella clarridgeiae carriage in stray cats from Ecuador and its link to a cat scratch disease outbreak in 2022.
Document Type
Academic Journal
Author
León-Sosa A; Instituto Nacional de Investigación en Salud Pública 'Leopoldo Izquieta Pérez', Dirección Técnica de Investigación, Desarrollo e Innovación, Guayaquil, Ecuador.; Orlando SA; Instituto Nacional de Investigación en Salud Pública 'Leopoldo Izquieta Pérez', Dirección Técnica de Investigación, Desarrollo e Innovación, Guayaquil, Ecuador; Universidad Espíritu Santo, Guayaquil, Ecuador.; Mora-Jaramillo N; Instituto Nacional de Investigación en Salud Pública 'Leopoldo Izquieta Pérez', Dirección Técnica de Investigación, Desarrollo e Innovación, Guayaquil, Ecuador.; Calderón J; Instituto Nacional de Investigación en Salud Pública 'Leopoldo Izquieta Pérez', Dirección Técnica de Investigación, Desarrollo e Innovación, Guayaquil, Ecuador.; Rodriguez-Pazmino AS; One Health Research Group, Universidad de Las Américas, Quito, Ecuador.; Carvajal E; One Health Research Group, Universidad de Las Américas, Quito, Ecuador.; Guizado-Herrera D; Instituto Nacional de Investigación en Salud Pública 'Leopoldo Izquieta Pérez', Dirección Técnica de Investigación, Desarrollo e Innovación, Guayaquil, Ecuador.; Narváez Y; Instituto Nacional de Investigación en Salud Pública 'Leopoldo Izquieta Pérez', Dirección Técnica de Investigación, Desarrollo e Innovación, Guayaquil, Ecuador.; Sánchez E; Instituto Nacional de Investigación en Salud Pública 'Leopoldo Izquieta Pérez', Dirección Técnica de Investigación, Desarrollo e Innovación, Guayaquil, Ecuador.; Arreaga A; Instituto Nacional de Investigación en Salud Pública 'Leopoldo Izquieta Pérez', Dirección Técnica de Investigación, Desarrollo e Innovación, Guayaquil, Ecuador.; Zevallos JC; Universidad Espíritu Santo, Guayaquil, Ecuador.; Carrillo C; Universidad Agraria del Ecuador, Guayaquil, Ecuador.; Garcia-Bereguiain MA; One Health Research Group, Universidad de Las Américas, Quito, Ecuador. Electronic address: magbereguiain@gmail.com.
Source
Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0370374 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6254 (Electronic) Linking ISSN: 0001706X NLM ISO Abbreviation: Acta Trop Subsets: MEDLINE
Subject
Language
English
Abstract
Introduction: The genus Bartonella includes species and subspecies of fastidious, facultative intracellular Gram-negative bacilli that infect a wide variety of mammalian reservoirs including cats and humans. In 2022, the Ecuadorian Ministry of Health reported an outbreak of cat scratch disease caused by B. henselae in the city of Guayaquil. Therefore, we aimed to characterize the presence of Bartonella spp. in domestic and stray cats from the area of Guayaquil where the outbreak happened in 2022.
Methods: Whole blood samples of 100 domestic and stray cats were collected. Riboflavin synthase (ribC) and 16S rRNA genes detection was performed by PCR using Bartonella spp. specific primers, followed by Sanger sequencing and phylogenetic analysis.
Results: 14 cats were positive for Bartonella spp. carriage. Phylogenetic analysis confirmed the presence of 12 cats infected with B. henselae and 2 cats with B. clarridgeiae.
Conclusions: There is a high prevalence of Bartonella spp. carriage in cats in the city of Guayaquil within the area where a recent cat scratch disease outbreak happened. Considering the high presence of cats and other domestic and stray animals in the city of Guayaquil, a One Health approach for surveillance and prevention of zoonotic diseases like cat scratch disease is needed.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Methods: Whole blood samples of 100 domestic and stray cats were collected. Riboflavin synthase (ribC) and 16S rRNA genes detection was performed by PCR using Bartonella spp. specific primers, followed by Sanger sequencing and phylogenetic analysis.
Results: 14 cats were positive for Bartonella spp. carriage. Phylogenetic analysis confirmed the presence of 12 cats infected with B. henselae and 2 cats with B. clarridgeiae.
Conclusions: There is a high prevalence of Bartonella spp. carriage in cats in the city of Guayaquil within the area where a recent cat scratch disease outbreak happened. Considering the high presence of cats and other domestic and stray animals in the city of Guayaquil, a One Health approach for surveillance and prevention of zoonotic diseases like cat scratch disease is needed.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)