학술논문
Absence of delayed chromosomal instability in a normal human fibroblast cell line after [sup 125] I iododeoxyuridine.
Document Type
Article
Author
Source
Subject
*CHROMOSOME abnormalities
*FIBROBLASTS
*RADIOISOTOPES
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Language
ISSN
0955-3002
Abstract
Purpose: To seek the delayed appearance of chromosomal abnormalities in human fibroblasts exposed to the Auger electron emitter [sup 125] I. ::Materials and methods: Normal untransformed human fibroblasts, HF19, were exposed to a concentration of [I[sup 125] ]IUdR, which allowed the survival of 37% of clonogens. Chromosomal analysis using both conventional Giemsa and fluorescence in situhybridization (FISH) was undertaken on non-clonal bulk cultures from 2 to 39 days after treatment. ::Results: The data show a declining level of unstable aberrations in the progeny of HF19 fibroblasts exposed to [I[sup 125] ]IUdR, eventually reaching control levels. ::Conclusions: The results provide evidence that [[sup 125] I]IUdR does not induce ongoing chromosomal instability in long-term culture, and gives further support to the use of Auger-electron emitting radionuclides in the treatment and diagnosis of tumours. [ABSTRACT FROM AUTHOR]