학술논문
Release of prostaglandin D by 2 murine mast cells: importance of metabolite formation for antiproliferative activity.
Document Type
Article
Source
Subject
*PROSTAGLANDINS
*MAST cells
*BONE marrow
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Language
ISSN
0962-9351
Abstract
PROSTAGLANDIN (PG) D , PGJ and Delta 12-PGJ are anti2 2 2 proliferative eicosanoids. We investigated the production of PGD by murine bone marrow-derived mast 2 cells (BMMC) taking into consideration metabolism of PGD to PGD and Delta 12-PGJ . PG-metabolites were quan2tified by 2high perform a 2nce liquid chromatography (HPLC) combined with radioimmunoassay (RIA). Stimulated with calcium ionophore A23187 BMMC released eight-fold more PGJ and Delta 12-PGJ than PGD . Conversion of endogeno2usly produced 2 2 PGD to PGJ and Delta 12-PGJ proceeded rapidly in 2 2 2 contrast to metabolism of exogenously added PGD . 2 The antiproliferative potency of these prostaglandins is demonstrated in vitro . We conclude that determination of PGD production by mast cells must take into 2 consideration rapid conversion to active derivatives, which may play a significant role in growth regulation. [ABSTRACT FROM AUTHOR]