학술논문
Molecularly imprinted electrochemical sensor for the ultrasensitive detection of cytochrome c.
Document Type
Academic Journal
Author
Campagnol D; Department of Molecular Sciences and Nanosystems, University Ca' Foscari of Venice, via Torino 155, 30172 Venice, Italy.; Karimian N; Department of Molecular Sciences and Nanosystems, University Ca' Foscari of Venice, via Torino 155, 30172 Venice, Italy. Electronic address: najmeh.karimian@unive.it.; Paladin D; Dott. Dino Paladin, bic incubatori Fvg, via Flavia 23/1, 34148 Trieste, Italy.; Rizzolio F; Department of Molecular Sciences and Nanosystems, University Ca' Foscari of Venice, via Torino 155, 30172 Venice, Italy; Pathology Unit, Centro di Riferimento Oncologico di Aviano (C.R.O.) IRCCS, 33081 Aviano, Italy.; Ugo P; Department of Molecular Sciences and Nanosystems, University Ca' Foscari of Venice, via Torino 155, 30172 Venice, Italy. Electronic address: ugo@unive.it.
Source
Publisher: Elsevier Country of Publication: Netherlands NLM ID: 100953583 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-562X (Electronic) Linking ISSN: 15675394 NLM ISO Abbreviation: Bioelectrochemistry Subsets: MEDLINE
Subject
Language
English
Abstract
Cytochrome c (Cyt c) is an important biomarker for the early stage of apoptosis that plays a role in the diagnosis and therapy of several diseases including cancer. Here, an electrochemical sensor based on molecularly imprinted polymer (MIP) for the ultrasensitive detection of Cyt c is studied. It is prepared by electropolymerization of o-phenylenediamine in the presence of Cyt c as template, followed by solvent extraction, resulting in the formation of Cyt c recognition sites. The MIP is characterised by cyclic voltammetry and differential pulse voltammetry, using ferrocenecarboxylic acid as redox probe. Voltammetric data indicates that the MIP-sensor behaves as an electrode with partially blocked surface. The partition isotherm obtained fits the Langmuir model, indicating a high affinity for Cyt c, with an association constant K a = 5 × 10 11 M -1 . DPV measurements allow to achieve extremely high analytical sensitivity and low detection limit, in the femtomolar range, with negligible unspecific adsorption. Satisfactory analytical recovery tests performed in the presence of possible interfering proteins and in diluted human serum confirmed the selectivity of the MIP-sensor as well as its potential applicability for real samples analysis.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier B.V. All rights reserved.)