학술논문
Attenuated Total Reflectance–Fourier Transform Infrared Spectroscopy Coupled with Multivariate Analysis for Measurement of Acesulfame-K in Diet Foods.
Document Type
Article
Author
Source
Subject
*REFLECTANCE
*OPTICAL reflection
*FOURIER transform infrared spectroscopy
*FOURIER transform spectroscopy
*MULTIVARIATE analysis
*ANALYSIS of variance
*ACESULFAME-K
*DIET
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Language
ISSN
0022-1147
Abstract
Fourier transform infrared (FTIR) spectroscopy was investigated as a method for analysis of acesulfame-K content after a simple extraction procedure for certain commercial diet food samples. Partial least squares (PLS) models were developed for prediction of acesulfame-K using select spectral ranges on the basis of relevant IR absorption bands associated with acesulfame-K. The acesulfame-K content of test food samples was predicted accurately in the fingerprint region between 1100 and 1300 cm−1 with a maximum prediction error of 9.82% when compared with conventional HPLC method. The PLS was found to be a consistently better predictor when both PLS and principal component regression (PCR) analyses were used for quantification of acesulfame-K. The developed procedure was further validated by comparing with HPLC results as well as recovery studies. As a quick tool, the method developed is expected to be used for routine estimation of acesulfame-K in commercial products. [ABSTRACT FROM AUTHOR]