학술논문
Impact of HIV-1 Genetic Diversity on Plasma HIV-1 RNA Quantification.
Document Type
Article
Author
François Rouet; Chaix, Marie-laure; Nerrienet, Eric; Ngo-Giang-Huong, Nicole; Plantier, Jean-Christophe; Burgard, Marianne; Peeters, Martine; Damond, Florence; Koumavi Ekouevi, Didier; Msellati, Philippe; Ferradini, Laurent; Rukobo, Sandra; Maréchal, Valérie; Schvachsa, Nilda; Wakrim, Lahcen; Rafalimanana, Christian; Rakotoambinina, Benjamin; Viard, Jean-Paul; Seigneurin, Jean-Marie; Rouzioux, Christine
Source
Subject
*POLYMERASE chain reaction
*REVERSE transcriptase
*VIROLOGY
*HIV infections
*EPIDEMICS
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Language
ISSN
1525-4135
Abstract
The article describes the development of a second generation of long terminal repeat-based real-time reverse transcriptase-polymerase chain reaction (RT-PCR) in the Virology Laboratory at Necker Hospital in Paris, France using a reduced length minor groove binder probe. According to the authors, RT-PCR assay accurately quantified relevant HIV-1 subtypes and circulating recombinant forms with epidemic significance.