학술논문
Molecular identification of Crimean-Congo haemorrhagic fever virus in Hyalomma rufipes and Amblyomma variegatum in the Upper East Region of Ghana.
Document Type
Article
Author
Addo, Seth Offei; Bentil, Ronald Essah; Addae, Charlotte Adwoa; Baako, Bernice Olivia Ama; Yartey, Kevin Nii; Ansah‑Owusu, Jane; Tawiah-Mensah, Christopher Nii Laryea; Agbodzi, Bright; Kumordjie, Selassie; Yeboah, Clara; Asoala, Victor; Dunford, James C.; Larbi, John Asiedu; Baidoo, Philip Kweku; Wilson, Michael David; Diclaro II, Joseph W.; Dadzie, Samuel K.
Source
Subject
Language
ISSN
0304-8608
Abstract
Sampled ticks were screened for Crimean-Congo haemorrhagic fever virus (CCHFV) using an assay that targets the nucleoprotein gene region of the S segment, a conserved region of the CCHFV genome. Minimum infection rates of 0.34% and 0.10% were obtained when testing pools of Hyalomma rufipes and Amblyomma variegatum, respectively. Next-generation sequencing and phylogenetic analysis showed that the S and L segments of the CCHFV isolate clustered with those of similar isolates of genotype III. However, analysis of the M segment showed that reassortment had occurred, causing this segment to cluster with those of isolates of genotype I, providing the first evidence of such an occurrence in Ghana. [ABSTRACT FROM AUTHOR]