학술논문
Predictive Potential of RNA Polymerase B (II) Subunit 1 (RPB1) Cytoplasmic Aggregation for Neoadjuvant Chemotherapy Failure.
Document Type
Article
Author
Nagy-Mikó, Bence; Németh-Szatmári, Orsolya; Faragó-Mészáros, Réka; Csókási, Aliz; Bognár, Bence; Ördög, Nóra; Borsos, Barbara N.; Majoros, Hajnalka; Ujfaludi, Zsuzsanna; Oláh-Németh, Orsolya; Nikolényi, Aliz; Dobi, Ágnes; Kószó, Renáta; Sántha, Dóra; Lázár, György; Simonka, Zsolt; Paszt, Attila; Ormándi, Katalin; Pankotai, Tibor; Boros, Imre M.
Source
Subject
*NEOADJUVANT chemotherapy
*RNA polymerase II
*HEAT shock proteins
*RNA polymerases
*CD30 antigen
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Language
ISSN
1661-6596
Abstract
We aimed to investigate the contribution of co-translational protein aggregation to the chemotherapy resistance of tumor cells. Increased co-translational protein aggregation reflects altered translation regulation that may have the potential to buffer transcription under genotoxic stress. As an indicator for such an event, we followed the cytoplasmic aggregation of RPB1, the aggregation-prone largest subunit of RNA polymerase II, in biopsy samples taken from patients with invasive carcinoma of no special type. RPB1 frequently aggregates co-translationally in the absence of proper HSP90 chaperone function or in ribosome mutant cells as revealed formerly in yeast. We found that cytoplasmic foci of RPB1 occur in larger sizes in tumors that showed no regression after therapy. Based on these results, we propose that monitoring the cytoplasmic aggregation of RPB1 may be suitable for determining—from biopsy samples taken before treatment—the effectiveness of neoadjuvant chemotherapy. [ABSTRACT FROM AUTHOR]