부산대학교 도서관

Histopathological and micrometric alterations of developing chick testes and adrenal were explored. There were selected 110 fertilized eggs, equally divided into two groups named as Vehicle control (Vc) and Bifenthrin (BF) group respectively. Technical grade bifenthrin was injected in the eggs (1mg/kg) using Normal Saline (NS)+DMSO interphase as vehicle in BF group whereas the Vc eggs likewise received injections of NS+DMSO only. The embryos were incubated 14 days in an incubator and finally removed from the egg shells. The embryos were weighed and fixed in 70% alcohol solution for further studies. The results have shown various histological alterations in developing chick testes such as decrease in the number of stem (529 µm2) was significantly lower in BF group (3.45±0.47) as compared to Vc group (13.80 ±0.62) and interstitial cells, mean enhancement in luminal space of seminiferous tubules, dramatic reduction in wall thickness of seminiferous tubules, clumping of interstitial cells and reduced number of sertoli cells. Moreover, significant alterations in mean wall thickness of seminiferous tubule, mean interstitial cell count around the seminiferous tubules, mean CSA of luminal space of seminiferous tubules of testicular parameters were observed. Likewise, histological alteration of BF exposure in developing chick adrenal medulla was overall shrinkage, focal degenerations, loose connective tissue network and for adrenal cortex mainly cellular hypertrophy was noted. Additionally, significant micrometric variations in developing adrenal medulla for mean medullary region size, mean cellular count parameters and adrenal cortex for mean cortex size, mean cell count, mean cortical cellular size parameters were noted. These findings show that BF is highly toxic to developing testes and adrenal in chick embryos indicating its developmental endocrine disruption potentials. It is thus suggested that this otherwise frequently used insecticide should be avoided as much as possible. [ABSTRACT FROM AUTHOR]