학술논문
Dermal Real‐Time Quaking‐Induced Conversion Is a Sensitive Marker to Confirm Isolated Rapid Eye Movement Sleep Behavior Disorder as an Early α‐Synucleinopathy.
Document Type
Article
Author
Kuzkina, Anastasia; Panzer, Celine; Seger, Aline; Schmitt, Daniela; Rößle, Jonas; Schreglmann, Sebastian R.; Knacke, Henrike; Salabasidou, Elena; Kohl, Antonia; Sittig, Elisabeth; Barbe, Michael; Berg, Daniela; Volkmann, Jens; Sommer, Claudia; Oertel, Wolfgang H.; Schaeffer, Eva; Sommerauer, Michael; Janzen, Annette; Doppler, Kathrin
Source
Subject
Language
ISSN
0885-3185
Abstract
Background: Skin biopsy is a potential tool for the premortem confirmation of an α‐synucleinopathy. Objective: The aim was to assess the aggregation assay real‐time quaking‐induced conversion (RT‐QuIC) of skin biopsy lysates to confirm isolated rapid eye movement sleep behavior disorder (iRBD) as an α‐synucleinopathy. Methods: Skin biopsies of patients with iRBD, Parkinson's disease (PD), and controls were analyzed using RT‐QuIC and immunohistochemical detection of phospho‐α‐synuclein. Results: α‐Synuclein aggregation was detected in 97.4% of iRBD patients (78.4% of iRBD biopsies), 87.2% of PD patients (70% of PD biopsies), and 13% of controls (7.9% of control biopsies), with a higher seeding activity in iRBD compared to PD. RT‐QuIC was more sensitive but less specific than immunohistochemistry. Conclusions: Dermal RT‐QuIC is a sensitive method to detect α‐synuclein aggregation in iRBD, and high seeding activity may indicate a strong involvement of dermal nerve fibers in these patients. © 2023 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society. [ABSTRACT FROM AUTHOR]