부산대학교 도서관

• 38 strains of endophytic fungi were isolated from silver birch. The presence of Betulin and Betulinic acid was confirmed in 32 isolates. • The highest amount of betulin in Fusarium cicatricum (311.604 mg/ml) and Penicillium citrinum (409/381 mg/ml) and the highest amount of Betulinic acid in Alternaria alternate (431.275 mg/ml) was found. • Cellulose nanofibers increased the amount of terpenes by 27.5 and 8 times respectively in P. citrinum and F. fuckelii compared to the control. This study specifically aims to produce Betulin and Betulinic acid derivatives, which are anticancer terpenes by endophytic fungi isolated from the bark of silver Birch (Betula pendula Roth) for the first time. Betulin can be converted or decomposed into its derivatives over time, but all these derivatives are known, as terpenes how, are exciting prospects for application in medicine and cosmetics. In order to carry out this research, bark samples were collected from silver birch trees in Siahmarzkooh – Golsetan – Iran. Samples were cultured in potato dextrose agar (PDA) after surface sterilization. Thirty-eight endophytic fungi were isolated. The ability of Betulin and Betulinic acid induction by endophytic fungi was measured by HPLC and three isolates with the highest amount were selected and molecularly recognized (PCR-sequencing). In the next step, elicitors were induced into 2-year-old mycelium reserves of birch endophytic fungi. The cultures were extracted with three concentrations and three different times of cellulose nanofibers and sodium nitrate, and the changes of terpenes were measured by HPLC. The results showed that 32 of 38 isolates have terpene. Fusarium fuckelii (311.604 µg/mL), Penicillium citrinum (381/409 µg/mL) had the highest amount of Betulin, and Alternaria alternate (431.275 µg/mL) had the highest amount of Betulinc acid. The results reconfirmed the conversion of Betulin into its derivatives over time. Cellulosic nanofibers had a more significant effect on the increase of terpene as an elicitor, so 100 mg/L of it induced 27.5 and 8 times in P. citrinum and F. fuckelii , respectively, compared to the control. This study showed that the endophytic fungi isolated from the silver birch trees could produce, increase terpenes, and probably can be more easily used in the cosmetic or pharmaceutical industries. [ABSTRACT FROM AUTHOR]