학술논문
Molecular Heterogeneity of Pediatric AML with Atypical Promyelocytes Accumulation in Children—A Single Center Experience.
Document Type
Article
Author
Borkovskaia, Aleksandra; Bogacheva, Sofia; Konyukhova, Tatiana; Dadakhanova, Elina; Gaskova, Marina; Soldatkina, Olga; Dubrovina, Maria; Popov, Alexander; Mikhailova, Ekaterina; Inushkina, Evgenia; Kazanov, Marat; Matveev, Evgeniy; Novichkova, Galina; Maschan, Michael; Maschan, Alexey; Olshanskaya, Yulia; Zerkalenkova, Elena
Source
Subject
*ACUTE promyelocytic leukemia
*ACUTE myeloid leukemia
*CHROMOSOME abnormalities
*GENETIC variation
*CHROMOSOMAL translocation
*NUCLEOTIDE sequencing
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Language
ISSN
2073-4425
Abstract
Acute promyelocytic leukemia (APL) pathogenesis is based on RARA gene translocations, which are of high importance in the diagnosis of and proper therapy selection for APL. However, in some cases acute myeloid leukemia (AML) demonstrates APL-like morphological features such as atypical promyelocytes accumulation. This type of AML is characterized by the involvement of other RAR family members or completely different genes. In the present study, we used conventional karyotyping, FISH and high-throughput sequencing in a group of 271 de novo AML with atypical promyelocytes accumulation. Of those, 255 cases were shown to carry a typical chromosomal translocation t(15;17)(q24;q21) with PML::RARA chimeric gene formation (94.1%). Other RARA-positive cases exhibited cryptic PML::RARA fusion without t(15;17)(q24;q21) (1.8%, n = 5) and variant t(5;17)(q35;q21) translocation with NPM1::RARA chimeric gene formation (1.5%, n = 4). However, 7 RARA-negative AMLs with atypical promyelocytes accumulation were also discovered. These cases exhibited TBL1XR1::RARB and KMT2A::SEPT6 fusions as well as mutations, e.g., NPM1 insertion and non-recurrent chromosomal aberrations. Our findings demonstrate the genetic diversity of AML with APL-like morphological features, which is of high importance for successful therapy implementation. [ABSTRACT FROM AUTHOR]