부산대학교 도서관

AIM: Although impact of metabolic changes on neutrophil count is well defined, how changes in neutrophil count would affect lipid metabolism was not investigated. Thus, aim was to evaluate impact of changes in neutrophil number on blood cholesterol levels. METHODS: To establish neutropenia and neutrophilia, two different types of recombinant G-CSF and anti-neutrophil serum (ANS) was subcutaneously injected into both young C57BL/6 male(n=12) and female(n=12) mice. To examine the possible underlying mechanism of relationship between neutrophil count and LDL levels, blood IL-17, G-CSF and liver HMG-CoA reductase enzyme activity were examined in male mice. To further evaluate the impact of sexes, blood samples were collected from male (n=12) and female (n=13) human subjects at two different time points (menstruation and late-follicular phase). RESULTS: While any significant changes in neutrophil numbers increased total blood cholesterol levels in male mice, an increased total blood cholesterol level (p=0.0339) was only occurred with increasing neutrophil count (p=0.006) in female mice. Moreover, it was demonstrated that elevation in total cholesterol level in male mice was associated with LDL (p=0.0008), but not HDL. There was a significant correlation between total cholesterol and LDL and neutrophils only in female mice (p=0.0113, p=0.0242 respectively), but not in males. In consistence with mice data, it was demonstrated in female subjects that when estrogen levels were significantly increased during menstrual cycle (p=0.0091), neutrophil numbers were significantly decreased (p=0.0034), and cholesterol levels were significantly increased (p=0.0325). And while there were no significant differences in G-CSF levels, a significant increase in IL-17 was observed only in ANS-given mice(p=0.0486), and there was a significant decrease in HMG-CoA Reductase activity in both ANS-(p=0.006) and filgrastim-injected mice(p=0.0096) when compared to the control group. CONCLUSONS: Concequently, it was demonstrated that any significant changes in neutrophil count directly affect LDL levels. Further, this interaction was observed during the menstrual cycle with fluctuating estrogen levels. [ABSTRACT FROM AUTHOR]