학술논문
Evaluation of cellular response to Clostridium difficile toxin-A: a network analysis.
Document Type
Article
Author
Source
Subject
*GUT microbiome
*BOTULINUM toxin
*PROTEINS
*ONCOGENES
*NUCLEAR proteins
*AUTOPHAGY
*MOLECULAR pathology
*PHOSPHATASES
*HEAT shock proteins
*SIGNAL peptides
*BIOINFORMATICS
*GENE expression
*CLOSTRIDIUM diseases
*DESCRIPTIVE statistics
*EPITHELIAL cells
*DATA analysis software
*VASCULAR endothelial growth factors
*TRANSCRIPTION factors
*CARRIER proteins
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Language
ISSN
2008-2258
Abstract
Aim: The current study aimed to determine crucial genes targeted by toxin-A through network analysis. Background: Clostridium difficile (C difficile) produces toxin-A and toxin-B and is known as a risk factor for hospital infection, especially after broad spectrum antibiotic therapy. Bioinformatics findings have led to the introduction of a set of genes and biological terms that are targeted by toxin-B in colon epithelia. Methods: The significant differentially expressed genes (DEGs) of human intestinal Caco-2 cells treated by toxin-A versus control were retrieved from gene expression omnibus (GEO). The queried DEGs were analyzed using by protein-protein interaction (PPI) network analysis through STRING database and Cytoscape software v.3.7.2. Results: Among 157 significant DEGs, JUN, VEGFA, CDKN1A, ATF3, SNAI1, DUSP1, HSPB1, MCL1, KLF4, FOSL1, HSPA1A, and SQSTM1 were determined as hubs and JUN, DUSP1, DUSP5, EZR, MAP1LC3B, and SQSTM1 were highlighted as bottlenecks. Conclusion: JUN, DUSP, and SQSTM1 are possible drug targets to prevent and treat C difficile infection. [ABSTRACT FROM AUTHOR]