학술논문
Receptor-Mediated Basic Fibroblast Growth Factor Signaling Regulates Cyclic Pressure-Induced Human Endothelial Cell Proliferation.
Document Type
Article
Source
Subject
*VASCULAR endothelium
*FIBROBLAST growth factors
*GROWTH factors
*MITOGENS
*ENDOTHELIUM
*ENDOTHELIAL seeding
*BLOOD vessels
*MONOCLONAL antibodies
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Language
ISSN
1062-3329
Abstract
Vascular endothelial cells sense and respond to pressure by molecular mechanism(s) which, to date, remain poorly understood. The present study investigated basic fibroblast growth factor (bFGF) signaling as a putative mechanotransduction pathway involved in the proliferative responses of human umbilical vein endothelial cells (HUVECs) to 60/20 mm Hg cyclic pressure at 1 Hz for 24 h. Under these conditions, the enhanced proliferative response of these HUVECs was not associated with an increased synthesis/release of bFGF, but involved rapid (within 30 min from the onset of exposure to pressure) tyrosine phosphorylation of the bFGF receptor, FGFR-2, Furthermore, monoclonal antibodies to either bFGF or FGFR-2 attenuated the increased proliferation of HUVECs exposed to 60120 mm Hg cyclic pressure. HUVECs proliferation under 60/20 mm Hg at 1 Hz cyclic pressure is, therefore, dependent upon bFGF and involves FGFR-2 activation. [ABSTRACT FROM AUTHOR]